M.bovis.org overview
• Patterns 1903
• Highest SB number SB2478
• Last update May 2017

FAQ

Mbovis.org

What is Mbovis.org database?

Mbovis.org is a free database for the naming of spoligotype patterns from strains of the animal-adapted (RD9 deleted) lineage of the MTBC.

How do I submit a new spoligotype pattern?

Search for the spoligotype pattern in the database. If the pattern is not in the database, a message will be displayed asking for the e-mail to send you the instructions to submit the new patterns.

How do I cite Mbovis.org database?

Smith NH., Upton P. "Naming spoligotype patterns for the RD9-deleted lineage of the Mycobacterium tuberculosis complex; www.Mbovis.org". Infect Genet Evol. 12(4):873-6. 2012.


DOI: 10.1016/j.meegid.2011.08.002

Which strains are included in Mbovis.org?

See this link for information about the strains included.

Which nomenclature for spoligotypes is used in Mbovis.org?

See this link for information about the spoligotype nomenclature.

What is the binary (BIN) code?

The spoligotype binary code uses the binary number system being 0 negative (spacer missing) and 1 positive (spacer present).


Example:


Original pattern
Binary code 1 0 0 1 1 1 1 1 0 1 1 1 1 1 1 0 1 1 1 1 0 1 1 1 1 1 1 1 1 1 0 0 0 0 1 1 0 1 0 0 0 0 0
Written as 1001111101111110111101111111110000110100000

What is the hexadecimal (HEX) code?

Spoligotype patterns can be named using the HEX code system (Dale et al., 2001). The binary spoligotype pattern is divided up into 6 blocks each containing 7 or 8 spacers:


1 - 7, 8 - 14, 15 - 21, 22 - 28, 29 - 36 and 37- 43


The binary pattern of each blocks is converted to a two digit hexadecimal number. The 6X2 digit code is used as the name of the spoligotype pattern.


Example:


Original pattern
Binary code 1 0 0 1 1 1 1 1 0 1 1 1 1 1 1 0 1 1 1 1 0 1 1 1 1 1 1 1 1 1 0 0 0 0 1 1 0 1 0 0 0 0 0
Hex blocks 1001111 1011111 1011110 1111111 11000011 0100000
Hex code 4F 5F 5E 7F C3 20
Written as 4F-5F-5E-7F-C3-20

What is the octal (OCT) code?

Spoligotype patterns can be named using the OCT code system (Dale et al., 2001). The pattern is read in groups of 3, as a 3-digit binary number can be read as a single digit octal number; in this way the binary series 000, 001, 010, 011, 100, 101, 110, 111 is read respectively as 0, 1, 2, 3, 4, 5, 6, 7. Following 14 groups of 3, the 43rd spacer is read on its own, simply as 0 or 1.


Example:


Original pattern
Binary code 1 0 0 1 1 1 1 1 0 1 1 1 1 1 1 0 1 1 1 1 0 1 1 1 1 1 1 1 1 1 0 0 0 0 1 1 0 1 0 0 0 0 0
Octal blocks 100 111 110 111 111 011 110 111 111 111 000 011 010 000 0
Octal code 4 7 6 7 7 3 6 7 7 7 0 3 2 0 0
Written as 476773677703200

Navigating this website constitutes acceptance of the use of cookies by third parties and own, necessary for proper operation